PENGARUH SUPLEMENTASI DAN PROTEKSI MINYAK
BIJI KAPUK TERHADAP FERMENTABILITAS RUMINAL RUMPUT GAJAH
SECARA IN
VITRO DENGAN CAIRAN RUMEN SAPI
(The Effect of Supplementation and Protection
of Kapok Seed Oil on In vitro Ruminal Fermentability of Elephant Grass on
Cattle Rumen Fluids)
D. D. Dinata, Widiyanto dan R. I.
Pujaningsih
Fakultas
Peternakan dan Pertanian Universitas Diponegoro
Kampus
drh. R. Soejono Koesomowardojo, Tembalang Semarang 50275
dessdonndinn@gmail.com
ABSTRAK
Tujuan penelitian adalah mengetahui
interaksi antara suplementasi dan proteksi minyak biji kapuk (MBK) terhadap
kecernaan dan hasil fermentasi ruminal rumput gajah secara in vitro. Materi yang digunakan
adalah rumput gajah, minyak biji kapuk, reagen KOH dan CaCl2 serta
cairan rumen sapi. Rancangan percobaan
yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial 3 x 5 dengan
2 kali ulangan, faktor I yaitu aras suplementasi MBK : 5% (S1); 10% (S2) dan
15% (S3), faktor II yaitu aras proteksi : 0% (P0); 25% (P1); 50% (P2); 75% (P3)
dan 100% (P4), serta perlakuan kontrol tanpa suplementasi dan proteksi
(S0P0). Hasil penelitian menunjukkan
bahwa tidak ada interaksi antara suplementasi dan proteksi terhadap KcBK dan
KcBO. Nilai KcBK dan KcBO tertinggi
dihasilkan oleh perlakuan kontrol, yaitu 55,17% dan 54,27%. Hasil penelitian menunjukkan ada interaksi
antara suplementasi dan proteksi terhadap produksi VFA dan NH3. Produksi VFA dan NH3 tertinggi
dicapai pada aras suplementasi 10% dan proteksi 25% yaitu 152,5 dan 6,50
mM. Simpulan penelitian adalah
suplementasi dan proteksi minyak biji kapuk tidak mempengaruhi hasil fermentasi
ruminal, namun menurunkan kecernaan ransum basal rumput gajah secara in vitro.
Kata kunci: minyak biji kapuk, suplementasi, proteksi, in vitro.
ABSTRACT
The purpose of this research was to
study the interaction between supplementation and protection of kapok seed oil
(KSO) influence on in vitro
digestibility and ruminal fermentability products of elephant grass. Material used were elephant grass, kapok seed
oil, KOH and CaCl2 reagens and cattle rumen fluids. Completely randomized design (CRD) was used with
two factorial pattern 3 x 5 with 2 replications, first factor was supplementation
of KSO consist of 3 levels: 5% (S1); 10% (S2); and 15% (S3), respectively. Second factor was protection consist of 5
levels: 0% (P0); 25% (P1); 50% (P2); 75% (P3) and 100% (P4); also control
treatment without supplementation and protection of KSO (S0P0). The result of this research showed there was
no interaction between supplementation and protection of KSO on digestibility
of elephant grass. The highest In vitro Dry Matter Digestibility
(IVDMD) and In vitro Digestibility of
Organic Material (IVDOM) reached by control treatment, 55,17% and 54,27%,
respectively. The conclusion of this
research showed infulence of interaction between supplementation and protection
of KSO on VFA and NH3 production.
The highest VFA and NH3 production reached by 10%
supplementation and 25% protection were 152,5 and 6,50 mM. The conclusion recommended that supplementation
and protection of KSO was not influenced ruminal fermentation, but decreased on
in vitro digesbility of elephant
grass.
Keyword: kapok seed oil, supplementation,
protection, in vitro.